Composite

Part:BBa_K2926283:Design

Designed by: Astrid T�bber   Group: iGEM19_Bielefeld-CeBiTec   (2019-10-21)


Basic Insert to express Cas13a (Lwa) and a guideRNA array in S. cerevisiae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4744
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4769
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1780
    Illegal BglII site found at 3460
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4744
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4744
    Illegal AgeI site found at 105
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2859


Design Notes

alvnly,


Source

adlv,

References